THE RELATIONSHIP OF SPERM PRODUCTIVITY AND SEX ACTIVITY OF DIFFERENT GENOTYPE BREEDING BOARS BY ACTN1 AND FSHβ GENES

Abstract

The article is devoted to the estimation of breeding boars’ genotypes  according to  the  indices  of sex
activity and sperm productivity and identification of the desired ACTN1, FSHβ  gene genotypes for further
breeding work in the herd. The purpose of our research was to identify the relationship of breeding boars’
genetic structure by ACTN1, FSHβ genes and describe the effect of their polymorphism on the indices of
sperm productivity and sex activity of boars belonging to different breeds. The analysis of sperm productivity
by ACN1 gene showed that the AA genotype of boars had a larger ejaculate volume by 28.6 ml (11.8 %,
P≤0.05)  than  BB  genotype  boars of the same age, but were inferior  to AB genotype boars  by 52.4 ml
(19.3 %). It was found out that sperm productivity and sex activity of breeding boars as to FSHβ gene were
ambivalent: boars of the Large  White breed had a larger ejaculate  volume (89.0–33.7 ml, or  by  0.8–4.3
times), and breeding boars of Landrace breed, on the contrary, were inferior  as to  this  index  (114.5–
123.3 ml less, or by 51.5–57.7 %). The results are statistically highly reliable (p≤0.001). On the average as
to the estimated boars, a significant difference was established between the genotypes concerning the sper-
matozoon  motility  (p≤0.05).  A  statistically  significant  preference  (P≤0.001)  of  heterotic  boars of AB
genotype, ACTN1 gene of the Large White breed, Landrace, and Durok breeds was revealed as to the indices
of contact with phantom and erection by 42.5–55.4 sec., as well as ejaculation intensity by 0.082–0.313 sec.,
respectively, which is confirmed by higher indices (2.1–4.2). AccordIng to the FSHβ gene, the same tendency
regardIng sex activity of the studied boars was confirmed. On the whole, the analysis of haplotypes of ACN1
and FSHβ genes has shown that the selection of boars for their libido and sperm productivity will be more
effective in AVAA sub-gene, as compared with AVAV and AAAV genotypes, where there is an increase of
ejaculate  by 7.6–38.6 %,  spermatozoon  concentration  –  by  14.3–30.2 %, spermatozoon  motility by 2.5–
4.6 %,  and  higher  mating  activity: by the  contact of breeding boar  with phantom and erection by 47.8–
79.2 %, and ejaculation  intensity – by 5.0–14.5 %. To assess the productive qualities of breeding boars, a
separate role has been noted for using sperm productivity and sex activity indices, which make it possible to
carry out the objective estimation of boars’ breedIng value for determining their breedIng rating.