INFLUENCE OF NATIVE LIPOSOMES AND “MEMBRANOSTABIL” PREPARATION ON THE CONTENT OF SOME BLOOD SERUM PROTEIN FRACTIONS IN NEWBORN CALVES

Abstract

The results of applying “Membranostabil” developed by the authors and native liposomes from
phospholipid bilayer on the soy lecithin base for the correction of albumins and haptoglobin content value
indices in the blood serum of newborn calves during the period of colostral immunity formation have been
presented. Albumins and haptoglobin content values in the blood serum of newborn calves have been studied
in dynamics – from the 1st day after birth to the 11 days of age. The investigation was carried out in 3 groups
of newborn calves of Ukrainian local dairy breed – the control and two experimental. The level of serum
protein fractions was investigated by the method of polyacrylamide gel electrophoresis. The quantitative
assessment of protein fractions was carried out by electrophoregram scanning with their subsequent graphic
reconstruction and by relative units or area calculation using computer program. It was established, that
“Membranostabil” and native liposomes from phospholipid bilayer on the soy lecithin base activated the
transportation of albumins and haptoglobin in the small intestine and led to reliable growth of their content
in comparison with calves in the control group. The dynamics with comparative analysis of albumin and
haptoglobin content values in the blood serum between the indices in calves from separate groups  was
shown. More pronounced growth of albumins and haptoglobin content under the influence of the used native
liposomes in the blood of newborn calves was demonstrated. More pronounced effect of “Membranostabil”
preparation on the own albumin synthesis by liver cells was found. The preventive effect of native liposomes
and “Membranostabil” preparation on developing pathological changes in the experimental calves’
organism was found. It was established that during the period from the first to the third day of calves’ life in
every group, the level of haptoglobin in their blood serum dropped drastically with the following increase on
the seventh day. Several hypotheses concerning probable reasons of haptoglobin level decreasing in the
blood serum of newborn calves on the third day of life were expressed. Lower haptoglobin content (as a
protein of an acute phase with immune modulating properties) was found in the blood serum of calves in
experimental groups on the 11th day of life in comparison with animals in the control group, which ensures
the prevention of the second phase of early immune deficiency, sepsis, the development of digestion disorders
and other young animal diseases.