Особливості отримання щільної фекальної культури яєць гельмінтів роду Trichuris, виділених від овець

Анотація

Наведено результати досліджень щодо ефективності запропонованого способу отримання щільної фекальної культури яєць нематод роду Trichuris, виділених від овець. Ефективність запропонованого способу за різних режимів відстоювання фекальної суспензії (10, 15 та 20 хв.) відносно показника кількості отриманих яєць нематод перевищує флотаційно-центрифужний та загальновідомий методи на 30,44–56,55 % та 12,65–45,44 % відповідно. Удосконалений спосіб дає змогу скоротити витрати часу на проведення методики порівняно із флотаційно-центрифужним (на 8,28–25,03 %) та загальновідомим методами (на 4,70–16,77 %).

The results of research on the effectiveness of the proposed method for obtaining a dense faecal of nematodes’ eggs of Trichuris genus in sheep are presented.

The effectiveness of our method for different modes of settling the faecal suspension (10, 15 and 20 min) concerning to the number of nematode eggs obtained exceeds the flotation-centrifugal and well-known methods by 30.44–56.55% and 12.65–45.44% respectively. The improved method allows to reduce time loss on conducting the procedure compared with flotation-centrifuge (by 8.28–25.03%) and well-known methods (4.70–16.77%).

For the purpose of successful fight against parasitosis, scientists of the world annually develop and produce a large number of drugs and formulations of chemical and biological origin that are pre-tested with a lot of approbation. One of such approbation is the use of experimental parasitosis simulation in the conditions of in vitro.

Egg nature of causative agents of parasitic diseases is often used in the development of new methods for lifelong diagnosis and conducting comparative effectiveness of existing ones. However, in the literature, insufficient information has been found on the methods for obtaining eggs of helminths.

In this regard, the aim of the research is to improve the method of obtaining a dense fecal eggs of worms of the Trichuris genus in sheep. The research task is to determine the effectiveness of the proposed method in comparison with the well-known ones.

The research has been conducted on the base of the Laboratory of Parasitology and Veterinary-Sanitary Expertise at Poltava State Agrarian Academy.

The eggs of trichuroses are obtained in three ways: a flotation-centrifuge method for cultivating eggs of Trichuris muris; a well-known method of cultivating eggs Trichuris muris; the proposed method with different terms for fecal suspension (10, 15 and 20 min).

By testing a flotation-centrifuge method for obtaining trichurise eggs from sheep, it has been found that the average number of washed eggs is 15.72±0.67. Using a well-known method, it was found that the average obtained number of eggs is 19.74±0.74 specimens. The use of our method for different modes of settling the fecal suspension
(10, 15 and 20 min) resulted in an average of 22.66±0.59, 36.18±1.50 and 28.10±0.90 eggs.

Conclusions:

1. The improved method has a higher efficiency in terms of the number of eggs obtained compared to flotation-centrifuge (30.44 to 56.55%) and well-known (12.65 to 45.44%) methods.
2. It has been found out that the efficiency of the offered method exceeds the efficiency of the flotation-centrifugal (by 8.28–25.03%) and the well-known (on 4.70–16.77%) methods in terms of time loss.