Species differentiation of animal chlamidiosis agents by means of polimerase chain reaction

Abstract

Ten PCR test systems have been developed for the species differentiation of the Chlamydia genus bacteria, which are etiological factors of mammalian and bird chlamydioses, namely C. abortus, C. avium, C. caviae, C. galinaceae, C. muridarum, C. felis, C. pecorum, C. pneumoniae, C. psittaci and C. suis. The basis includes ten constructed and synthesized pairs of oligonucleotide primers flanking different sized DNA fragments of the main outer membrane protein gene (MOMP) of chlamydias. Specificity of the developed PCR test systems is confirmed by the results obtained using the site «Bio.bsu.by» and the «Blast» computer software and by the results of the PCR method studies on 17 samples of biological materials, 11 of which being samples of the Chlamydia genus bacteria’s control DNA, and 6 ones being DNA samples of leptospiras and babeses.