Determination of the effects of the bactericidal effect on E. coli bacteria of the new disinfectant "Diolayd", its phenol coefficient and protein index

Abstract

cheap means is urgent. The purpose of the work was to determine the time exposure, effective bactericidal
concentration, lack of bacteriostatic effect of the new disinfectant "Diolide" for its action on the test culture
E. coli ATSS 25922; determination of phenolic coefficient and protein index. Determination of bacteriostatic
properties and optimal bactericidal concentration of Diolaid was carried out with working solutions of 0.04
(100 mg/dm3 of chlorine dioxide); 0.06 (150 mg/dm3); 0.1 (250 mg/dm3) and 0.16 % (400 mg/dm3) after their
action on E. coli ATCC 25922 during exposures of 30 and 60 min by the suspension method with washing of
bacteria from the disinfectant, subsequent cultures and their accounting. The phenolic coefficient was
determined by the method of serial dilutions of "Diolaide" and phenol from a concentration of 1:50 to 1:2834.7
for effects on E. coli ATCC 25922 for 10 and 30 minutes. The protein coefficient was determined with E. coli
ATCC 25922 at exposures of 10 and 30 min, by the method of double serial dilutions of "Diolaide" alone and
in combination with inactivated blood serum to simulate protein contamination. Methods: microscopic,
cultural, biochemical, statistical. According to the analysis of the results of research on the dilution of
"Diolaid" in concentrations of 0.06; 0.1 and 0.16 (%) for exposures of 30 and 60 min showed an effective
bactericidal effect in the absence of a bacteriostatic effect, as evidenced by the complete absence of growth of
E. coli ATCC 25922 on the media during the incubation period of 48 and 72 h, with its intensive growth in
controls It was established that the phenolic coefficient of "Diolaide" corresponded to the indicator of 12.7.
The protein index was 2.76 units, testifying that due to protein contamination of the surfaces intended for
"Diolaid" disinfection, its bactericidal efficiency decreased by 2.76 times. Therefore, the working solutions of
"Diolide" at concentrations of 0.06% (150 mg/dm3 of chlorine dioxide) and higher for 30 minutes or longer
provided complete neutralization of E. coli ATCC 25922 without the manifestation of a bacteriostatic effect.
The phenolic coefficient of "Diolaide" was 12.7 times higher than that of phenol. The protein index "Diolaide"
confirmed a decrease in bactericidal efficiency due to protein pollution by 2.76 times.