THE EFFECT OF Α2-ADRENOCEPTOR AGONISTS ON THE BODY OF LABORATORY MICE WITH ITS LONG-TERM USE

Abstract

Virtually all drugs used in anesthesia in both veterinary and human medicine, to some extent, have a variety of side effects on body functions. This is what motivates scientists to search for and implement new, safer and more effective schemes of combined anesthesia. To this end, new anesthetics undergo a large number of trials and tests, and only then they are launched on the pharmaceutical market. In particular, the safety of such drugs is relevant in severe cases, when the animal needs long-term administration. Therefore, this study aimed to determine the effect of Sedazine and Dexmedetomidine with their long-term use on the general clinical condition and functions of the internal organs of white mice, and the changes they cause. The work was performed on the basis of the Regional State Laboratory of the State Food and Consumer Service in Poltava region. To study the effects of Sedazin (Biovet Bulavi, Poland) and Dexmedetomidine (Orion Pharma, Finland) on the functions of internal organs and changes that occur under their influence with prolonged use, two groups of white mice of the same age group were formed. Mice in both groups were administered intramuscular anesthetics at a dose of 0.1 ml once a day for five consecutive days: Sedazine in the control group; experimental - Dexmedetomidine. During the experiment, animals of both groups were observed, in particular: coordination of mouse movements; changes in the respiratory function of the body (the number of respiratory movements and their depth), metabolic parameters (changes in appetite, problems with the act of defecation or urination); the time spent waking the animal. Monitoring of vital signs was performed in 3 stages: 1) before drug administration; 2) 15 minutes after administration; 3) after waking up. Studies show that long-term use of Dexmedetomidine increases the frequency of respiratory movements in mice by an average of 5% with each passing day, and Sedazin – by 6.7%, which may indicate a negative effect of these drugs with long-term use on respiratory function. It was found that the time of awakening with each subsequent administration of drugs lengthens, with the use of Dexmedetomidine – by 8 %, and Sedazine – by 8.4 %. Also in both groups there were problems with appetite (towards its reduction) and the act of defecation, which is a sign of deterioration of the general condition of animals.